The weeks shown in color represent the start and end points of chemical exposure for each study.

Study Results Study Details References

Results for Chlorpyrifos

Varied effects on adenylyl cyclase activity

Subjects: Zivic Miller Lab rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 mg/kg bw/d

Other doses tested: 5 mg/kg on pnd 11-14

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: 5 and 10 days of age

Reference [PubMed Link]
Auman JT, Seidler FJ, Slotkin TA. 2000. Neonatal chlorpyrifos exposure targets multiple proteins governing the hepatic adenylyl cyclase signaling cascade: implications for neurotoxicity. Developmental Brain Research 121(1):19-27.
Study Results Study Details References

Results for Chlorpyrifos

Decreased body weight

Subjects: Unspecified strain rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 or 5.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: post-natal day 5 through 20

Reference [PubMed Link]
Campbell CG, Seidler FJ, Slotkin TA. 1997. Chlorpyrifos interferes with cell development in rat brain regions. Brain Res Bull 43(2):179-189.
Study Results Study Details References

Results for Chlorpyrifos

Increased mortality at 5 mg/kg

Subjects: Unspecified strain rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 or 5.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: post-natal day 1 through 4

Reference [PubMed Link]
Campbell CG, Seidler FJ, Slotkin TA. 1997. Chlorpyrifos interferes with cell development in rat brain regions. Brain Res Bull 43(2):179-189.
Study Results Study Details References

Results for Chlorpyrifos

Decreased total induced adenylyl cyclase activity in the liver of males at post-natal day 30; increased glucagone, NaF and forskolin induced activity of adenylyl cyclase in the liver of males at post-natal day 60; decreased Mn2+ induced activity of adenylyl cyclase in the liver of females at post-natal day 60

Subjects: Sprague-Dawley rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: post-natal day 5, 10, 30 and 60

Reference [PubMed Link]
Meyer A, Seidler FJ, Slotkin TA. 2004. Developmental effects of chlorpyrifos extend beyond neurotoxicity: critical periods for immediate and delayed-onset effects on cardiac and hepatic cell signaling. Environ Health Perspect 112(2):170-178.
Study Results Study Details References

Results for Chlorpyrifos

Increased levels of thiobarbituric acid-reactive species

Subjects: Sprague-Dawley rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 or 5.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: post-natal day 5

Reference [PubMed Link]
Slotkin TA, Oliver CA, Seidler FJ. 2005. Critical periods for the role of oxidative stress in the developmental neurotoxicity of chlorpyrifos and terbutaline, alone or in combination. Developmental Brain Research 157(2):172-180.
Study Results Study Details References

Results for Chlorpyrifos

Increased mortality at 5 mg/kg

Subjects: Sprague-Dawley rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 or 5.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected sub-cutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: post-natal day 5

Reference [PubMed Link]
Slotkin TA, Levin ED, Seidler FJ. 2006. Comparative developmental neurotoxicity of organophosphate insecticides: effects on brain development are separable from systemic toxicity. Environ Health Perspect 114(5):746-751.
Study Results Study Details References

Results for Chlorpyrifos

Decreased heart basal adenylyl cyclase activity in the presence and absence of G-proteins (GTP) at 1 mg/kg; increased heart M2-receptor binding at 5 days of age at 5 mg/kg; decreased heart weight, β-receptor binding, and adenylyl cyclase activity in the presence of GTP at 5 days of age at 5 mg/kg

Subjects: Sprague-Dawley rats

Chemical: Chlorpyrifos

Low doses tested: 1.0 or 5.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected sub-cutaneously

Exposure duration: post-natal day 1 – post-natal day 4 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 25)

Age of measurement: 5 and 10 days of age

Reference [PubMed Link]
Song X, Seidler FJ, Saleh JL, Zhang J, Padilla S, Slotkin TA. 1997. Cellular mechanisms for developmental toxicity of chlorpyrifos: Targeting the adenylyl cyclase signaling cascade. Toxicology & Applied Pharmacology 145(1):158-174.
Study Results Study Details References

Results for Chlorpyrifos

Decreased body weight

Subjects: Unspecified strain rats

Chemical: Chlorpyrifos

Low doses tested: 2.0 mg/kg bw/d

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – single dose (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 21)

Age of measurement: post-natal day 1

Reference [PubMed Link]
Whitney KD, Seidler FJ, Slotkin TA. 1995. Developmental neurotoxicity of chlorpyrifos: Cellular mechanisms. Toxicology & Applied Pharmacology 134(1):53-62.
Study Results Study Details References

Results for Chlorpyrifos

Increased mortality at 5 mg/kg at post-natal day 1

Subjects: Unspecified strain rats

Chemical: Chlorpyrifos

Low doses tested: 1.0, 2.0 or 5.0 mg/kg bw/d

Other doses tested: 11, 22, 45 mg/kg bw/d

Route of administration: dissolved in DMSO and injected subcutaneously

Exposure duration: post-natal day 1 – single dose (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 21)

Age of measurement: post-natal day 1 and 8

Reference [PubMed Link]
Whitney KD, Seidler FJ, Slotkin TA. 1995. Developmental neurotoxicity of chlorpyrifos: Cellular mechanisms. Toxicology & Applied Pharmacology 134(1):53-62.
Study Results Study Details References

Results for Chlorpyrifos

Decreased body weight gain in the high dose regimen

Subjects: Sprague-Dawley rats

Chemical: Chlorpyrifos

Low doses tested: 1.0, or 1.0 and 2.0, or 1.5 and 3.5 mg/kg bw/d

Route of administration: dissolved in corn-oil and given by gavage

Exposure duration: post-natal day 1 – post-natal day 8 (comparable to human prenatal development from approximately day 1 of week 20 to day 1 of week 29)

Age of measurement: post-natal day 4 and 8

Reference [PubMed Link]
Guo-Ross SX, Chambers JE, Meek EC, Carr RL. 2007. Altered muscarinic acetylcholine receptor subtype binding in neonatal rat brain following exposure to chlorpyrifos or methyl parathion. Toxicol Sci 100(1):118-127.
Study Results Study Details References

Results for Chlorpyrifos

Decreased body weight on post-natal day 46

Subjects: Sprague-Dawley rats

Chemical: Chlorpyrifos

Low doses tested: 1 mg/kg bw/d increasing to 4 mg/kg bw/d

Route of administration: dissolved in corn oil and given by oral gavage

Exposure duration: post-natal day 1 – post-natal day 20 (comparable to human prenatal development from approximately day 1 of week 20 to beyond birth)

Age of measurement: during adolescence

Reference [PubMed Link]
Johnson FO, Chambers JE, Nail CA, Givaruangsawat S, Carr RL. 2009. Developmental Chlorpyrifos and Methyl Parathion Exposure Alters Radial-Arm Maze Performance in Juvenile and Adult Rats. Toxicol Sci 109(1):132-142.
Study Results Study Details References

Results for Chlorpyrifos

Decreased body weight gain at 1.0 and 1.35 mg/kg.

Subjects: Wistar rats

Chemical: Chlorpyrifos

Low doses tested: 0.01, 1.0 or 1.35 mg/kg bw/d

Route of administration: dissolved in corn oil and delivered via oral gavage

Exposure duration: post-natal day 1 – post-natal day 20 (comparable to human prenatal development from approximately day 1 of week 20 to beyond birth)

Age of measurement: post-natal day 21

Reference [PubMed Link]
Mansour SA, Mossa AH. 2010. Adverse effects of lactational exposure to chlorpyrifos in suckling rats. Human & Experimental Toxicology 29(2):77-92.
Study Results Study Details References

Results for Chlorpyrifos

Decreased relative kidney weight at 1.0 and 1.35 mg/kg and altered levels of kidney marker parameters.

Subjects: Wistar rats

Chemical: Chlorpyrifos

Low doses tested: 0.01, 1.0 or 1.35 mg/kg bw/d

Route of administration: dissolved in corn oil and delivered via oral gavage

Exposure duration: post-natal day 1 – post-natal day 20 (comparable to human prenatal development from approximately day 1 of week 20 to beyond birth)

Age of measurement: post-natal day 21

Reference [PubMed Link]
Mansour SA, Mossa AH. 2010. Adverse effects of lactational exposure to chlorpyrifos in suckling rats. Human & Experimental Toxicology 29(2):77-92.
Study Results Study Details References

Results for Chlorpyrifos

Decreased relative liver weight at 1.0 and 1.35 mg/kg and altered levels of liver marker parameters.

Subjects: Wistar rats

Chemical: Chlorpyrifos

Low doses tested: 0.01, 1.0 or 1.35 mg/kg bw/d

Route of administration: dissolved in corn oil and delivered via oral gavage

Exposure duration: post-natal day 1 – post-natal day 20 (comparable to human prenatal development from approximately day 1 of week 20 to beyond birth)

Age of measurement: post-natal day 21

Reference [PubMed Link]
Mansour SA, Mossa AH. 2010. Adverse effects of lactational exposure to chlorpyrifos in suckling rats. Human & Experimental Toxicology 29(2):77-92.