The weeks shown in color represent the start and end points of chemical exposure for each study.
|Study Results||Study Details||References|
Results for Phthalates (BBP and DBP)Increased DNA damage, phospho-ATM, p-p53 ser15, p-p53 ser 20, cyclin-dependent kinase inhibitor (p-21), Bax levels, Apaf-1 levels, cytochrome c levels, mitochondrial fraction of cleaved -caspase 9 and cleaved-caspase 3 in MC3T3-E1 osteoblasts at 1 μM benzyl butyl phthalate (BBP) and di-n-butyl phthalate (DBP) at various times between 30 minutes and 24 hours; decreased c-myc, EDK4, cyclin D3, and phospho - Rb ser 780 at 1 μM BBP at various times between 30 minutes and 24 hours
Subjects: Harlan Sprague Dawley ICR (CD-1) mice
Chemical: Phthalates (BBP and DBP)
Low doses tested: 1.0 μM
Route of administration: in vitro culture
Exposure duration: approximately 1 day of age – 24 hours in vitro culture (comparable to human prenatal development from approximately day 1 of week 15 to day 1 of week 26)
Age of measurement: 30 minutes to several days in treatment culture
|Reference [PubMed Link]
Sabbieti MG, Agas D, Santoni G, Materazzi S, Menghi G, Marchetti L. 2009. Involvement of p53 in phthalate effects on mouse and rat osteoblasts. J Cell Biochem 107(2):316-327.